A simple spectrofluorometric estimation of psoralen from Psoralea corylifolia (l.) (Bavchi) seeds

A simple, accurate ,precise, sensitive spectrofluorometric method was developed for estimation of psoralen in methanolic extract of Psoralea corylifolia seeds. It showed strong intense blue fluorescence having excitation and emission wavelength 290nm and 427.66 nm, respectively. Linear relationship for the fluorescence intensity was obtained in the range 50-100 μg /mL. Limit of detection and limit of quantification was found to be 6.51 μg /mL and 19.75 μg /mL, respectively. The method was statistically validated and found suitable for estimation of psoralen in a methanol extract of plant Psoralea corylifolia.